Stab motility media with inoculating needle Property it tests for: This test is done to help differentiate species of bacteria that are motile. It shows the fermentation pattern of glucose and lactose through a pH indicator and the production of hydrogen sulfide gas by reaction with ferrous salts in the media.
Voges-Proskauer Procedures The unknown culture was then inoculated on the agar plates and test tubes to culture and grow the unknown microorganism using best standard practices. In the case of Mannitol Salt Agar the streak plate technique was used by performing the following procedures. An inoculating loop is sterilized by flaming it using a Bunsen burner.
The inoculating loop is cooled down.
The cap of the unknown culture was removed from the top of the tube and was also flamed for sterilization. The inoculating loop was inserted in the unknown tube to pick up culture. The the top part of the tube was then passed again over the flame.
The culture is streak on the plate using the streak plate technique and aseptic technique. Similar method was used on the test tubes without using the streak plate method. That is, culture was picked up using the aseptic technique and introduced on each of the test tubes. After these procedures, the culture was disposed and the inoculated plates and test tubes were placed in the refrigerator until the next period.
Second Period and Results.Microbiology 20 Biochemical Unknown – Spring (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May regardbouddhiste.com of a possible 70 points you.
Objectives. Aim of this experiment is to differentiate between the two major categories of bacteria, gram positive and gram negative. Through this experiment, gram staining skills develop.
We are starting our "unknown" lab this week and we each will get 2 types of bacteria that we need to identify on our own. I plan on doing a gram stain for each, then do streak plates w/selective media.
The intent of this report is to identify Unknown # The conclusion of Unknown #12’s identity was reached after a series of tests and subcultures were conducted using aseptic techniques. Aseptic techniques are necessary in order to avoid contamination of the culture being examined. Dr. Burne.
Sep 08, · How to Write a Microbiology Lab Report. Whether you're studying microbiology in high school or as a college student, you'll need to write a number of lab reports.
If you used an unknown microorganism in the experiment, record the number, letter, or identifying characteristic of the microorganism. Also state%(7). Unknowns – 1 IDENTIFICATION OF UNKNOWNS In this exercise, you will draw upon many of your new skills to identify unknown bacteria.
You will be given three bacterial cultures and asked to identify each to the level of genus.